-
The Immune Cell Landscape in Kidneys of Lupus Nephritis Patients
Internal Dataset- Authors
- Arnon AraziDeepak A. RaoCeline BerthierAnne Davidson39 more author(s)...
- Description
Lupus nephritis is a potentially fatal autoimmune disease for which the current treatment is ineffective and often toxic. To develop mechanistic hypotheses of disease, we analyzed kidney samples from patients with lupus nephritis and from healthy control subjects using single-cell RNA sequencing. Our analysis revealed 21 subsets of leukocytes active in disease, including multiple populations of myeloid...
- Subject
-
KidneyLupus NephritisRNA-SeqSequence Analysis, RNA
- Access Rights
- Free to all with registrationApplication Required
- Local Expert
- Anne DavidsonArnon AraziBetty Diamond
-
NBQX disodium salt, an AMPA receptor antagonist, is used for an in vitro treatment of human smooth muscle cells
Internal Dataset- Authors
- Alessandro GallinaUrszula RykaczewskaRobert WirkaApril Caravaca12 more author(s)...
- Description
Vascular smooth muscle cells (VSMCs) are key constituents of both normal arteries and atherosclerotic plaques. They have an ability to adapt to changes in the local environment by undergoing phenotypic modulation. The amino-acid glutamate has been associated with CVD risk and VSMCs metabolism in experimental vascular research, and glutamate receptors regulate VSMC biology and promote pulmonary vascular...
- Subject
-
GlutamatesMyocytes, Smooth MuscleRNA-SeqSequence Analysis, RNA
- Access Rights
- Free to All
- Local Expert
- Peder Olofsson
-
Identification of grade and origin specific cell populations in serous epithelial ovarian cancer by single cell RNA-seq
Internal Dataset- Authors
- Andrew ShihAndrew MenzinJill WhyteJohn Lovecchio5 more author(s)...
- Description
Here we investigated different cell populations within ovarian cancer using single-cell RNA seq: fourteen samples from nine patients with differing grades (high grade, low grade and benign) as well as different origin sites (primary and metastatic tumor site, ovarian in origin and fallopian in origin).
- Subject
-
CancerCarcinoma, Ovarian EpithelialOvarian NeoplasmsRNA-SeqSequence Analysis, RNA
- Access Rights
- Free to All
- Local Expert
- Andrew ShihAnnette LeePeter Gregersen
-
RNA-seq of DRG neurons isolated from alum vs. alum+KLH immunized mice
Internal Dataset- Authors
- Manojkumar GunasekaranProdyot ChatterjeeAndrew ShihGavin Imperato18 more author(s)...
- Description
Using a combination of rigorous molecular genetic analyses, transgenic mice, and adoptive transfer experiments, we demonstrate that DRGs do not synthesize these antigen-specific antibodies, but rather sequester primarily IgG1 subtype antibodies. As revealed by RNA-seq and targeted quantitative PCR (qPCR), dorsal root ganglion (DRG) sensory neurons harvested from either naïve or immunized mice lack...
- Subject
-
AntibodiesGanglia, SpinalInflammationRNA-SeqSensory Receptor CellsSequence Analysis, RNA
- Access Rights
- Free to All
- Local Expert
- Andrew ShihSangeeta Chavan
-
Prognostic Molecular Signatures and Biomarkers for Metastatic Progression in Stage I and II Clear Cell Renal Cell Carcinomas
Internal Dataset- Authors
- Andrew ShihNeal MurphyZachary KozelParas Shah8 more author(s)...
- Description
Presented is the differential expression of mRNA and miRNA in 44 Stage I and Stage II patients who developed metastasis within 5 years of nephrectomy, compared to 21 patients who remained disease free. Extracted RNA from nephrectomy specimens preserved in FFPE blocks was sequenced using RNAseq. MiRNA expression was done using the TaqMan OpenArray qPCR protocol.
- Subject
-
CancerMicroRNAsRNA-SeqSequence Analysis, RNA
- Access Rights
- Free to All
- Local Expert
- Andrew ShihAnnette Lee
